antibodies against cox 1  (Santa Cruz Biotechnology)

Journal: Journal of Sport and Health Science

Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice

doi: 10.1016/j.jshs.2025.101095

Figure Lengend Snippet: Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.

Article Snippet: Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

Techniques: Expressing, Western Blot, Isolation, Marker, Control, Membrane

Journal: Frontiers in Microbiology

Article Title: Qijiao Shengbai capsule ameliorates ulcerative colitis in mice by modulating gut microbiota and protecting the intestinal barrier, associated with suppression of the TLR4/MyD88/NF-κB pathway

doi: 10.3389/fmicb.2026.1875650

Figure Lengend Snippet: QJSB alleviates inflammation in DSS-induced UC mice in vivo ( n = 6). (A) , (B) , (C) and (D) Levels of MPO, IL-6, IL-1β, and TNF-α in the colon; (E) , (F) and (G) serum levels of IL-1β, IL-6, and TNF-α; (H) levels of LPS in feces. (I) protein blots for iNOS and COX-2; (J) Relative expression levels of iNOS; (K) relative expression levels of COX-2; (L) correlation analysis between inflammatory mediators and colon-specific metabolites. (### p < 0.001, ## p < 0.01, # p vs. Control; * p < 0.05, ** p < 0.01, *** p < 0.001vs. Model).

Article Snippet: TLR4, MyD88, IκBα, p-IκBα, NF-κB p65, NF-κB p-p65, iNOS, and COX-2 antibodies were obtained from Proteintech (Wuhan, China).

Techniques: In Vivo, Expressing, Analysis, Control

Journal: Cell Research

Article Title: Cold exposure aggravates vein occlusion through non-shivering thermogenesis-induced thrombocytopoiesis

doi: 10.1038/s41422-026-01280-2

Figure Lengend Snippet: a Diagram of lipolysis in an adipocyte in WT mice and Pnpla2 –/– mice. Pnpla2 encodes ATGL, a key intracellular lipase involved in the hydrolysis of stored fat and FFA release. Pnpla2 –/– mice are not able to liberate FFAs from adipose TG depots. b Weight quantification of BAT and WAT in WT mice and Pnpla2 –/– mice under 30 °C or 4 °C ( n = 4 samples per group). c Histological analysis of BAT and WAT in WT mice and Pnpla2 –/– mice under 30 °C or 4 °C. Quantification of adipocyte size ( n = 8 random fields per group). Scale bar: 100 μm. d Immunohistochemical staining of BAT and WAT with UCP1 and COX4, followed by counterstaining with DAPI (blue) in WT mice and Pnpla2 –/– mice under 30 °C or 4 °C. Quantification of UCP1 + signals and COX4 + signals ( n = 8 random fields per group). Scale bar: 50 μm. e c-FFA levels in WT mice and Pnpla2 –/– mice under 30 °C or 4 °C ( n = 4 mice per group). f PLT, PDW, and MPV analysis in PB from WT mice and Pnpla2 –/– mice under 30 °C or 4 °C ( n = 4 mice per group). g IP of C/EBPα in isolated BM MKs from WT mice and Pnpla2 –/– mice under 30 °C or 4 °C. Acetyl-C/EBPα was detected. Non-immune IgG was used as a negative control. h Thrombocytopoiesis-associated gene expression in isolated MKs from WT mice and Pnpla2 –/– mice under 30 °C or 4 °C ( n = 3 samples per group). * P < 0.05; ** P < 0.01; *** P < 0.001. NS not significant. Data are presented as mean ± SD.

Article Snippet: For immunohistochemical staining of adipose tissues, paraffin-embedded tissue sections were stained with a rabbit anti-UCP1 antibody (1:100; Cat# A5857; ABclonal) or a rabbit anti-COX4 antibody (1:100; Cat# A6564; ABclonal).

Techniques: Immunohistochemical staining, Staining, Isolation, Negative Control, Gene Expression